| Full Name |
THETM Anti-His [HRP] Monoclonal Antibody
|
Abbreviated name-1 |
His-tag Antibody [HRP],mAb, Mouse |
|
Description |
GenScript THETM Anti-His [HRP] Monoclonal Antibody is HRP conjugated THETM Anti-His Monoclonal Antibody (Mouse) (A00186) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays. |
Ig Subclass |
IgG1, k |
Storage |
THETM Anti-His [HRP] Monoclonal Antibody (Mouse) should be stored lyophilized until use. It is stable in lyophilized form for at least two years if stored at -20°C. The reconstituted antibody can be stored for up to three months at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles. |
Specificity |
THETM Anti-His [HRP] Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
Concentration |
1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
Clone |
6G2A9 |
Immunogen |
A synthetic peptide HHHHHH coupled to KLH |
Purification Method |
Protein A affinity column |
Reconstitution |
Reconstitute the lyophilized antibody (100 μg) with 100 μl of deionized water to the final concentration of 1 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
|
Conjugation |
Horseradish Peroxidase (HRP) |
Host Species |
Mouse |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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TECHNICAL MANUAL: 20070630080710 (PDF)![Mouse Anti His-tag [HRP] mAb](/site2/document/3375_20070711043948.JPG "THE<sup>TM</sup> Anti-His [HRP] Monoclonal Antibody")
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